FGF2 Protein

Produktnummer ABIN6699847

Hersteller Produkt- Nr.: 012-f01-u80-0010

Dieses Recombinant FGF2-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für FGF2 Protein (ABIN6699847)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein-Typ: Recombinant

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Güteklasse: Animal-Free

Produktdetails

Hersteller Produkt- Nr.: 012-f01-u80-0010

Hersteller: Rockland

Verwendungszweck: Rat Fibroblast Growth Factor-basic Recombinant Protein (Animal Free)

Aufreinigung: Fibroblast Growth Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis of reducing and non-reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of 3T3 cells and is typically less than 1 ng/mL

Anwendungsinformationen

Applikationshinweise:

Application Note: Fibroblast Growth Factor basic Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor basic in immunological assays. Lyophilized in Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer: Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: Fgf2

Hintergrund:

Synonyms: Heparin-binding growth factor 2 (HBGF-2), Prostatropin, Basic fibroblast growth factor (bFGF)

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Recombinant rat FGF-basic is a non-glycosylated protein, containing 146 amino acids, with a molecular weight of 16.4 kDa.

UniProt: P13109

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine