FGF1 Protein

Produktnummer ABIN6699828

Hersteller Produkt- Nr.: 009-001-u79-0010

Recombinant FGF1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für FGF1 Protein (ABIN6699828)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 97,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-u79-0010

Hersteller: Rockland

Verwendungszweck: Human Fibroblast Growth Factor acidic Recombinant Protein

Aufreinigung: Fibroblast Growth Factor acidic purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically 2.0 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Fibroblast Growth Factor acidic Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor acidic in immunological assays. Buffer formation: 10 mM sodium phosphate, 150 mM sodium sulfate, pH 7.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer: Buffer formation: 10 mM sodium phosphate, 150 mM sodium sulfate, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Andere Bezeichnung: FGF1

Hintergrund:

Synonyms: Heparin-binding growth factor 1 (HBGF-1), Beta-endothelial growth factor, ECGF-beta, acidic fibroblast growth factor (aFGF)

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-acidic, or FGF-1, is a particularly potent inducer of DNA synthesis and has chemotactic activities. Recombinant human FGF acidic is a non-glycosylated protein, containing 141 amino acids, with a molecular weight of 16 kDa.

UniProt: P05230

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung