G-CSF Protein

Produktnummer ABIN6699721

Hersteller Produkt- Nr.: 010-001-u93-0010

Dieses Recombinant G-CSF-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für G-CSF Protein (ABIN6699721)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-u93-0010

Hersteller: Rockland

Verwendungszweck: Mouse Granulocyte Colony Stimulating Factor Recombinant Protein

Aufreinigung: Granulocyte Colony Stimulating Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse M-NFS-60 cells and is typically less than 10-60 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Granulocyte Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Colony Stimulating Factor in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.01 M Sodium Citrate, pH 3.0

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Andere Bezeichnung: Csf3

Hintergrund:

Synonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietin

Background: Granulocyte Colony-Stimulating Factor, or G-CSF, is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant mouse G-CSF is a non-glycosylated protein, containing 179 amino acids, with a molecular weight of 19 kDa.

UniProt: P09920

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization