G-CSF Protein (AA 31-204)

Produktnummer ABIN666680

Dieses Recombinant G-CSF-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für G-CSF Protein (AA 31-204) (ABIN666680)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Activity Assay (AcA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 31-204

Sequenz: MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

Produktmerkmale: G-CSF, Human, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Andere Bezeichnung: G-CSF

Hintergrund: Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molekulargewicht: 18.8 kDa (175aa) confirmed by MALDI-TOF

NCBI Accession: NP_757373

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization