CXCL12 Protein

Produktnummer ABIN6699687

Hersteller Produkt- Nr.: 010-001-w22-0010

Recombinant CXCL12-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CXCL12 Protein (ABIN6699687)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-w22-0010

Hersteller: Rockland

Verwendungszweck: Mouse Stromal Cell-Derived Factor-1 alpha (CXCL12) Recombinant Protein

Aufreinigung: Stromal Cell-Derived Factor-1 alpha (CXCL12) purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity as determined by its ability to chemoattract human T cells at 50-100 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Stromal Cell-Derived Factor-1 alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stromal Cell-Derived Factor-1 alpha in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: Cxcl12

Hintergrund:

Synonyms: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 (TPAR1), C-X-C motif chemokine 12, Pre-B cell growth-stimulating factor (PBSF), Thymic lymphoma cell-stimulating factor (TLSF)

Background: Stromal Cell Derived Factor-1 alpha (SDF-1α), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1α signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human and mouse SDF-1α share 99 % sequence identity. Recombinant mouse SDF-1α is a non-glycosylated protein, containing 68 amino acids, with a molecular weight of 7.9 kDa.

UniProt: Q4FJL5

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling