Annexin V Protein (His tag)

Produktnummer ABIN6699527

Hersteller Produkt- Nr.: 009-001-r40s

Produktdetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Annexin V Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-r40s

Hersteller: Rockland

Verwendungszweck: Annexin 5 recombinant protein-HIS Epitope

Aufreinigung: Recombinant full-length human Annexin 5 Protein was expressed by E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Reinheit: >95%

Anwendungsinformationen

Applikationshinweise:

Application Note: Annexin 5 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol. Annexin 5 Protein is suitable for use in Western Blot. Expect a band approximately ~ 37 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: Annexin 5 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Andere Bezeichnung: ANXA5 (ANXA5 Produkte)

Hintergrund:

Synonyms: ANXA5, PP4, ANX5, ENX2, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha

Background: Annexin 5 is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. In vitro studies have shown that Annexin 5 is a cellular protein which plays a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin (1). Through affinity assays Annexin 5 has been localized on cells that expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death (2). Annexin 5 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Apoptosis/Autophagy, Cancer, and Neurobiology research.

NCBI Accession: NM_001154

Pathways: Apoptose