IL-4 Protein (AA 25-153)

Produktnummer ABIN6387871

Dieses Recombinant IL-4-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IL-4 Protein (AA 25-153) (ABIN6387871)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Activity Assay (AcA)

Reinheit: > 95% by SDS-PAGE

Produktdetails

Proteineigenschaft: AA 25-153

Sequenz: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.6ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL-4

Hintergrund: IL4, also known as Interleukin 4 isoform1, is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naive helper T-cell to Th2 cells. IL4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant human IL4, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 15 kDa (130aa)

NCBI Accession: NP_000580

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation