MAP4K3 Protein (AA 1-321) (His tag)

Produktnummer ABIN6387691

Dieses Recombinant MAP4K3-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für MAP4K3 Protein (AA 1-321) (His tag) (ABIN6387691)

Target: MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95% by SDS-PAGE

Produktdetails

Proteineigenschaft: AA 1-321

Aufreinigungstag / Konjugat: Dieses MAP4K3 Protein ist gelabelt mit His tag.

Sequenz: MTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL

Biological Activity Comment: Specific activity is > 70unit/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))

Andere Bezeichnung: glk

Hintergrund: Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 37.1 kDa (344aa) confirmed by MALDI-TOF

NCBI Accession: NP_416889

UniProt: P0A6V8

Pathways: MAPK Signalweg