Flagellin Protein (FliC) (Mutant) (His tag)

Produktnummer ABIN6253486

Dieses Recombinant Flagellin-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Flagellin Protein (FliC) (Mutant) (His tag) (ABIN6253486)

Target: Flagellin (FliC)

Protein-Typ: Recombinant

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % (SDS-PAGE)

Produktdetails

Proteineigenschaft: Mutant

Aufreinigungstag / Konjugat: Dieses Flagellin Protein ist gelabelt mit His tag.

Verwendungszweck: Flagellin (TLR5 Mutant) (rec.)

Spezifität: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

Kreuzreaktivität: Human, Maus

Produktmerkmale: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

Endotoxin-Niveau: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: Flagellin (FliC)

Andere Bezeichnung: Flagellin

Hintergrund:

FliC (TLR5 Mutant)

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.

Pathways: Inflammasome