Flagellin Protein (FliC) (Mutant) (His tag)
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- Target Alle Flagellin (FliC) Proteine anzeigen
- Flagellin (FliC)
- Protein-Typ
- Recombinant
- Proteineigenschaft
- Mutant
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Spezies
- Human, Maus
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses Flagellin Protein ist gelabelt mit His tag.
- Verwendungszweck
- Flagellin (TLR5 Mutant) (rec.)
- Spezifität
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
- Kreuzreaktivität
- Human, Maus
- Produktmerkmale
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
- Reinheit
- >95 % (SDS-PAGE)
- Endotoxin-Niveau
- <0.01EU/μg purified protein (LAL test).
- Biological Activity Comment
- Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
- Top Product
- Discover our top product FliC Protein
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Flagellin (FliC) proteinFliC Spezies: Borrelia burgdorferi Wirt: Escherichia coli (E. coli) Recombinant ELISA, WB
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Konzentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handhabung
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
- Haltbarkeit
- 6 months
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- Target
- Flagellin (FliC)
- Andere Bezeichnung
- Flagellin (FliC Produkte)
- Hintergrund
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FliC (TLR5 Mutant)
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.
- Pathways
- Inflammasome
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