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TUBB Protein (AA 170-419) (His tag)

TUBB Spezies: Human Wirt: Escherichia coli (E. coli) > 95 % AcA, CC Active
Produktnummer ABIN6239912
  • Target Alle TUBB Proteine anzeigen
    TUBB (Tubulin, beta (TUBB))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 170-419
    Spezies
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 37
    • 4
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses TUBB Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 95 %
    Biological Activity Comment
    Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MX1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TUBb with MX1.
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    Discover our top product TUBB Protein
  • Applikationshinweise
    Isoelectric Point: 6.6
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    TUBB (Tubulin, beta (TUBB))
    Andere Bezeichnung
    Tubulin Beta (TUBb) (TUBB Produkte)
    Hintergrund
    Alternative Names: TUB-B, TUBB5, TUBB1, M40
    Molekulargewicht
    32kDa
    UniProt
    P07437
    Pathways
    Microtubule Dynamics, M Phase
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