DKK1 Protein (AA 33-266) (His tag)

Produktnummer ABIN6239907

Produktdetails

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Biologische Aktivität: Active

Proteineigenschaft: AA 33-266

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses DKK1 Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: Dickkopf-related protein 1 (DKK1) is a protein which is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the CTNNb1 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. Besides, Catenin Beta 1 (CTNNb1) has been identified as an interactor of DKK1 thus a binding ELISA assay was conducted to detect the interaction of recombinant human DKK1 and recombinant human CTNNb1. Briefly, DKK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTNNb1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DKK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of DKK1 and CTNNb1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of DKK1 with CTNNb1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 8.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Andere Bezeichnung: Dickkopf Related Protein 1 (DKK1) (DKK1 Produkte)

Hintergrund: Alternative Names: SK, Dickkopf Homolog 1(Xenopus Laevis)

Molekulargewicht: 33kDa

UniProt: O94907

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation