Galectin 9 Protein (AA 1-355) (His tag,GST tag)

Produktnummer ABIN6239815

Dieses Galectin 9-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Galectin 9 Protein (AA 1-355) (His tag,GST tag) (ABIN6239815)

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Cell Culture (CC)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-355

Aufreinigungstag / Konjugat: Dieses Galectin 9 Protein ist gelabelt mit His tag,GST tag.

Produktmerkmale: Tag location: N-terminal His and GST Tag

Biological Activity Comment: GAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc). It is reported that GAL9 induces T-helper type 1 lymphocyte (Th1) death by binding to HAVCR2 (Hepatitis A virus cellular receptor 2); besides, the interaction between GAL9 and PDI (Protein disulfide-isomerase) leads to disulfide reductase activity increasing at the plasma membrane, therefore alters the plasma membrane redox state and enhances cell migration. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL9 with recombinant human HAVCR2 and recombinant human PDI separately. Briefly, GAL9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HAVCR2-coated and PDI-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GAL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of GAL9 with HAVCR2 and PDI were shown in Figure 1 and Figure 2, and this effect was in a dose dependent manner The binding activity of GAL9 with HAVCR2. Figure 2. The binding activity of GAL9 with PDI.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 9.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Andere Bezeichnung: Galectin 9 (GAL9)

Hintergrund: Alternative Names: LGALS9, Ecalectin, Lectin,Galactoside-Binding Soluble 9, Tumor antigen HOM-HD-21

Molekulargewicht: 69kDa

UniProt: O00182