Perforin 1 Protein (His tag,GST tag)

Produktnummer ABIN6120693

Dieses Recombinant Perforin 1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Perforin 1 Protein (His tag,GST tag) (ABIN6120693)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: > 80 %

Produktdetails

Aufreinigungstag / Konjugat: Dieses Perforin 1 Protein ist gelabelt mit His tag,GST tag.

Verwendungszweck: Human Perforin-1 (PRF1) Protein

Sequenz: KRSHKFVPG AWLAGEGVDV TSLRRSGSFP VDTQRFLRPD GTCTLCENAL QEGTLQRLPL ALTNWRAQGS GCQRHVTRAK VSSTEAVARD AARSIRNDWK VGLDVTPKPT SNVHVSVAGS HSQAANFAAQ KTHQDQYSFS TDTVECRFYS FHVVHTPPLH PDFKRALGDL PHHFNASTQP AYLRLISNYG THFIRAVELG GRISALTALR TCELALEGLT DNEVEDCLTV EAQVNIGIHG SISAEAKACE EKKKKHKMTA SFHQTYRERH SEVVGGHHTS INDLLF

Endotoxin-Niveau: < 1.0 EU per 1 μg (LAL method)

Biological Activity Comment: Active

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl and 5 % Trehalose.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin 1

Molekulargewicht: Calculated MW: 61.5 kDa
Observed MW (SDS-PAGE): 62 kDa

UniProt: P14222

Pathways: Apoptose, Caspase Kaskade in der Apoptose