P4HB Protein (AA 20-509) (His tag)
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- Target Alle P4HB Proteine anzeigen
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 20-509
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Spezies
- Maus
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Quelle
- Baculovirus infected Insect Cells
- Aufreinigungstag / Konjugat
- Dieses P4HB Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA AKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKN GDTASPKEYT AGREADDIVN WLKKRTGPAA TTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQ LDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANF EEVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDG AGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH
- Reinheit
- > 95 % by SDS - PAGE
- Endotoxin-Niveau
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.
- Top Product
- Discover our top product P4HB Protein
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Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) protein (GST tag)
P4HB Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 18-508) protein (His tag)P4HB Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (Active) protein (His tag)P4HB Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Andere Bezeichnung
- P4HB (P4HB Produkte)
- Hintergrund
- P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molekulargewicht
- 56.1kDa (498aa) 50-70KDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_035162
- UniProt
- P09103
- Pathways
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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