Interferon gamma Protein (IFNG) (AA 24-161)
Kurzübersicht für Interferon gamma Protein (IFNG) (AA 24-161) (ABIN7529268)
Target
Alle Interferon gamma (IFNG) Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 24-161
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Sequenz
- MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG
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Endotoxin-Niveau
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cytotoxicity assay using WiDr cells. The ED50 range ≤ 0.5ng/ml.
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Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag)
IFNG Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag,Biotin)IFNG Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (Active) proteinIFNG Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG Spezies: Maus Wirt: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG Spezies: Hamster Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG Spezies: Schwein Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (Active) protein (Fc Tag)IFNG Spezies: Ratte Wirt: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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Bioactivity Validated
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Liquid. In PBS buffer ( pH 7.4) containing 10 % glycerol, 1 mM DTT
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Interferon gamma (IFNG)
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Andere Bezeichnung
- IFN-gamma/IFNG
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Hintergrund
- IFNG is a member of the type II interferon family. This protein is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia. Recombinant human IFNG protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 16.3 kDa (139aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000610
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UniProt
- P01579
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Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie
Target
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