Histone H3.3 Protein (AA 1-136) (His tag)

Produktnummer ABIN5852959

Produktdetails

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-136

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Histone H3.3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Denatured

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Andere Bezeichnung: H3F3A (H3F3A Produkte)

Hintergrund: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family. Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molekulargewicht: 17.7kDa (159aa)

NCBI Accession: NP_002098

UniProt: P84243