IL-2 Protein

Produktnummer ABIN5505697

Dieses Recombinant IL-2-Protein wird in Insektenzellen exprimiert.

Kurzübersicht für IL-2 Protein (ABIN5505697)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Insektenzellen

Applikation: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Reinheit: Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-2 / IL2 protein expressed in Insect cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.5 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.0x10^6 units/mg, determined by the dose dependent stimulation of CTLL2 cell proliferation.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 10 mM NaH2PO4, pH 7.2, 150 mM NaCl.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: Interleukin-2 / IL2

Hintergrund: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 15.4 kDa

NCBI Accession: NP_000577

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation