WNT3A Protein (AA 19-352)

Produktnummer ABIN4948748

Dieses Recombinant WNT3A-Protein wird in Mammalian Cells exprimiert.

Kurzübersicht für WNT3A Protein (AA 19-352) (ABIN4948748)

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Mammalian Cells

Applikation: Functional Studies (Func)

Reinheit: 70-75 %

Produktdetails

Proteineigenschaft: AA 19-352

Spezifität: Stem cell or organoid culture

Aufreinigung: This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg)

Biological Activity Comment: Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.

Anwendungsinformationen

Applikationshinweise: 31-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA

Handhabung: Mix the protein by pipetting up and down but not by vortexing

Lagerung: -20 °C

Informationen zur Lagerung: Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.

Antigendetails

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Andere Bezeichnung: Wnt3a

Hintergrund: Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene

Molekulargewicht: 41 kDa evaluated by SDS-PAGE under reducing conditions

Gen-ID: 22416

NCBI Accession: NC_000077

UniProt: P27467

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity