Albumin (ALB) Protein

Details zu Produkt Nr. ABIN458790
Proteinname
Spezies
Schaf
51
16
11
9
8
6
5
5
4
3
2
1
1
1
1
1
1
1
1
Wirt
26
10
9
9
7
7
6
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Binding Studies (Bind), Dot Blot (DB), Haemagglutination (H), Immunodiffusion (ID), Immunoelectrophoresis (IEP), ELISA, Western Blotting (WB)
Optionen
Produktmerkmale Source: Sheep serum
Reinigung Chromatographically purified sheep albumin
Reinheit ≥ 98 % as determined by agar-gel electrophoresis. Tested in a concentration of 40 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques, this product contains a trace of an alpha-1 protein.
Proteinname
Hintergrund Albumin is a stable small polypeptide with a strong antigenicity. It has a molecular weight of 69,000 and an isoelectric point of 5.7. The protein is isolated from serum by sequential precipitation and further purified by ion exchange chromatography and affinity chromatography. The identity is confirmed and tested by immunoelectrophoresis and radial immuno-diffusion using antisera to total serum proteins and albumin.
Pathways Lipid Metabolism
Applikations-hinweise Purified sheep albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized, stable white powder.
Konservierungs-mittel Without preservative
Lagerung 4 °C/-20 °C
Informationen zur Lagerung This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. After opening the vial, the lyophilized content is reconstituted by adding 2 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.