CCDC40 Protein (AA 1-1192) (Strep Tag)

Produktnummer ABIN3136175

Produktdetails

Target: CCDC40 (Coiled-Coil Domain Containing 40 (CCDC40))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1192

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Aufreinigungstag / Konjugat: Dieses CCDC40 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MMDAEKVSTD GEAISEGEVG SNGETPPETE VEFIGETAPD TDVEFIGETS PGTDVEPTGE SIQETEVESI GEATPGMDVE PIKKTMTELN VESIGEETSE TDVDSIRKAL RGIDLESITV AYPPKKAKHR KVRPQAEVES TGRAAPEGEL EVSDHEKVEA LLDELDELSE IVSSPEVSYS DISPLEMGED DTNVSATSTD TFQQGIYEPI EPIEPTEPPE PAEPPKPAET PEDSTVRAPA HPYQRDFPMG ARHRFRLSIM GSLTPSDTDD LPLETDEPPQ QESVQSTPRA LEETRIQFLD QVQSLSPEAL LDRATEGSDE AEEEGSQLIV LDPDHPLMIR FQEALKGYLN RQMDKLKLDV QELDVATKQT RSQRQELGVN LYGVQQHLAR LQMQLEKSHD RHSLVACERR RKEEELQCAR SVYNKTCQTA NEERKKLAAL QTEVESLALH LFYMQNIEQD VRDDIQVMKQ VVRKTETEKM HAEVEKKKQD LFVDQLTERS HQLEENIALF EAQYLSQAED TRVLKKAVTE AITEIDTIAV EKKRILQQWT TSLVGMKHRN EAYKTVMDAL RECQHQVKST DSEIEVCKKS IMQEEEKNEK LARLLNRAET EATLVQKMTA QCLSKQEALQ TEFNTYQLAL QDTEEMLNKG YVEHSAVLSE LQATRQAFHQ EQELRQKMDM SMVDKLQEQG TSSKMTKYFH QLLRKLQKEN TNLVTHLSKI DGDIAQATLD ITNTNCKIDM HKKTLAEMDK EVKRFNDLIT NSESEIARRT ILIERKQSLI NFFNKQLEQM VSELGGEEAG PLELEIKRLS KLTEEYNTGV AEAQMTWLRL QQELVQVTHE REEQLVSVDQ LKKEVHIMEQ KKLRIESKIA HEKKEQKIVS RHMRDLDNDL SKLNMLLDKN RCSSEELEQN NIATETEFLR TLKDSERETI QMQEKLMELS EEKATLLNSF MEAEHQIMLW EKKIQLAKEM RSSVDSETGQ TEIRAMKAEI HRMKVRHGQL LKQQEKMIRD MELAVARRET IVVQAEGQSK IDKKVITKTE FHYQQRELQK KVREMHKATD DCTNTISELE ETQKFLSSSL QEKQQLLSEM QATTDVLEEE INQLTALKRQ NLLEIVTLQT RGKHLQAAIE GKYVFLHRNS RSQLMERKRL SVRLSQLNKV LSSVQEDYPQ YQEVLQSIQQ KIATKLETPE PS
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: CCDC40 (Coiled-Coil Domain Containing 40 (CCDC40))

Andere Bezeichnung: Ccdc40 (CCDC40 Produkte)

Hintergrund: Coiled-coil domain-containing protein 40 (Protein links),FUNCTION: Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC39 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39. {ECO:0000250|UniProtKB:A8IQT2, ECO:0000250|UniProtKB:Q4G0X9}.

Molekulargewicht: 136.8 kDa

UniProt: Q8BI79