Ttc41/Gnn Protein (AA 1-1318) (Strep Tag)

Produktnummer ABIN3135638

Produktdetails

Target: Ttc41/Gnn (Ttc41) (Tetratricopeptide Repeat Domain 41 (Ttc41))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1318

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Aufreinigungstag / Konjugat: Dieses Ttc41/Gnn Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MSDEASETGQ RYNGQPILKR QKPILPYICS TLDFQEERDF LAKSIFPRLN DICSSRGTYF KAVDLRWSAV KAHKSFTSNQ FRQYSCLQSQ HLKLSLDYVN RCFPFFIGLL GQTYGDFLPD YTPFLLSQVK DFESLSKGKK NLYIAAKNGY PWVLKTPNCS LTEFEIIQAV FRKKSQFQFF YFRTSNSLLR TFNEEEEEEE EKLSSAYLLN EQGKMKVGKL KAKIIGKGLP VRFYRDLEEL GDMVWKDWSA VVEKLYPFTT IMGNIDYKHS FENLYHEEFV ENCKQVFVTS KESNRTFEIL ERFAIKDLDL DLDTDSTIAG SGLDSILRIN SLPTCKSILL LSGERGCGKS TLIANWVSNF QSKHPGVLMI PYFVGSTCES CDIMSVIHYF VMELQHRANG PRLEMDFLNE DSNVLVFSLL VEVFIAAISL KPCILVLDGI EELIGIYGIS GQKAKDFSWL PRSLPPHCKF ILSSVSSSLS CKSLCARPDV KIVELNSIGD EDTKFNIFRQ HLSPADQERF GQSKPILRKK PNLSPLKLAI IASELQECKI YRNEFQCLRE YLEVASVQEL WELILKRWVE DYSWTLKPKD TTLDTVIPGP SGWVVDVLCL LCISHCGLAE DELLQLLDTM GYRDHHKVTA VHWAAFRQAT KTWIQEKPNG LLYFQHQSLR SAVEHKLLGV STPVRESNPN VAQNSVNHKK AHFHQVLMRF FQRQTIFWRV YQELPWHMKM SGYWEGLCNF ITNPSITDFI SKIQNPSLWT RLHLVHYWDV LLEAGNDVSE AFLLSVAKIE GEQFQKLKKR TTLSVLECSL SEITAADKGR IILFIGSFLK LMGKINEAEK LFLSAEDLLL QSPSMTEMLL RAQNAIGELY LEIGMTPKGL TYFQKAWSNL LRFTLSDLKI SQELMKQKVK VMNNLAESAP GEFLKENHVL EYATEISKYV TGNPRDHATM KYTEGVLMLA SGNAALAKLK FQECLTIRRW LFGNKNILVG EIMEFLADLL FFLLGENEKS QKKQAIEYYK QVIKIKEKAD TVATCKLVRK HLSISLSDTL CKLAGQLLSG DFCHHATMEA VSYLYRSLDL RAAHLGPTHA SIEGILHLLR EIQRSRGRRS WPQSMNHLFP NGSRNGFSLW ENVPKLNFHS AQSSDTVNTA MCMNIRRFQR VKSTQPSLVS DKPKYVPGKG KKTLAPILCK SAEEKFQRQA SDSQIWNSPR RQPARKKAAC PLKTVSLIDK NGLVRLSRQS VSSAELDSRK GLITSICRQP LQRPHNVDNP WKSISELVSE KWLFHTPQYC FTPQKPGFPR RSQIESKLLK TSDDPNKE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: Ttc41/Gnn (Ttc41) (Tetratricopeptide Repeat Domain 41 (Ttc41))

Andere Bezeichnung: Ttc41 (Ttc41 Produkte)

Hintergrund: Tetratricopeptide repeat protein 41 (TPR repeat protein 41) (Grp94-neighboring nucleotidase)

Molekulargewicht: 150.8 kDa

UniProt: Q692V3