PKMYT1 Protein (AA 1-490) (Strep Tag)

Produktnummer ABIN3129921

Dieses Recombinant PKMYT1-Protein wird in Cell-free protein synthesis (CFPS) produziert.

Kurzübersicht für PKMYT1 Protein (AA 1-490) (Strep Tag) (ABIN3129921)

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Cell-free protein synthesis (CFPS)

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Reinheit: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-490

Aufreinigungstag / Konjugat: Dieses PKMYT1 Protein ist gelabelt mit Strep Tag.

Sequenz: MTMPTEGTPP PLSGTPIPVP AYFRHAEPGF SLKRPGGLSR SLPPRPPAKG CIPVSRLFPP RTPGWHQPQP RRVSFLCETS EPLQSPGYDP SRPESFFQQN FQRLSRLGHG SYGEVFKVRS KEDGRLYAVK RYMSPFRGPK DRTRKLAEVG GHEKVGQHPH CVRLERAWEE GGILYLQTEL CGPSLQQHCE AWGASLPEAQ VWGYLRDILL ALDHLHSQGL VHLDVKPANI FLGPRGRCKL GDFGLLVELG SAGAGEAQEG DPRYMAPELL QGSYGTAADV FSLGLTILEV ACNMELPHGG EGWQQLRQGY LPPEFTAGLS SELRSVLAMM LEPDPQLRAT AEALLALPML RQPRPWNVLW YMAAEALSRG WALWQALVTL LCWLWHGLVH PASWLQPPGP PATPPGSPPC SPLLDSTLSS SWDNDSIGPS LSPETVLSRI TRRTSTPRGR YIPRDALDLT DVDSEPPRGP CPTFEPRNLL SLFEDSLDPA
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Andere Bezeichnung: Pkmyt1

Hintergrund: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC 2.7.11.1) (Myt1 kinase),FUNCTION: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. {ECO:0000250|UniProtKB:Q99640}.

Molekulargewicht: 54.1 kDa

UniProt: Q9ESG9

Pathways: Mitotic G1-G1/S Phases, M Phase