Cyclin B3 Protein (CCNB3) (AA 1-1395) (Strep Tag)

Produktnummer ABIN3090976

Produktdetails

Target: Cyclin B3 (CCNB3)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1395

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Cyclin B3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MLLPLPPQSS KPVPKKSQSS KIVPSHHDPS EKTGENCQTK ISPSSLQESP SSLQGALKKR SAFEDLTNAS QCQPVQPKKE ANKEFVKVVS KKINRNTHAL GLAKKNKRNL KWHKLEVTPV VASTTVVPNI MEKPLILDIS TTSKTPNTEE ASLFRKPLVL KEEPTIEDET LINKSLSLKK CSNHEEVSLL EKLQPLQEES DSDDAFVIEP MTFKKTHKTE EAAITKKTLS LKKKMCASQR KQSCQEESLA VQDVNMEEDS FFMESMSFKK KPKTEESIPT HKLSSLKKKC TIYGKICHFR KPPVLQTTIC GAMSSIKKPT TEKETLFQEL SVLQEKHTTE HEMSILKKSL ALQKTNFKED SLVKESLAFK KKPSTEEAIM MPVILKEQCM TEGKRSRLKP LVLQEITSGE KSLIMKPLSI KEKPSTEKES FSQEPSALQK KHTTQEEVSI LKEPSSLLKS PTEESPFDEA LAFTKKCTIE EAPPTKKPLI LKRKHATQGT MSHLKKPLIL QTTSGEKSLI KEPLPFKEEK VSLKKKCTTQ EMMSICPELL DFQDMIGEDK NSFFMEPMSF RKNPTTEETV LTKTSLSLQE KKITQGKMSH LKKPLVLQKI TSEEESFYKK LLPFKMKSTT EEKFLSQEPS ALKEKHTTLQ EVSLSKESLA IQEKATTEEE FSQELFSLHV KHTNKSGSLF QEALVLQEKT DAEEDSLKNL LALQEKSTME EESLINKLLA LKEELSAEAA TNIQTQLSLK KKSTSHGKVF FLKKQLALNE TINEEEFLNK QPLALEGYPS IAEGETLFKK LLAMQEEPSI EKEAVLKEPT IDTEAHFKEP LALQEEPSTE KEAVLKEPSV DTEAHFKETL ALQEKPSIEQ EALFKRHSAL WEKPSTEKET IFKESLDLQE KPSIKKETLL KKPLALKMST INEAVLFEDM IALNEKPTTG KELSFKEPLA LQESPTYKED TFLKTLLVPQ VGTSPNVSST APESITSKSS IATMTSVGKS GTINEAFLFE DMITLNEKPT TGKELSFKEP LALQESPTCK EDTFLETFLI PQIGTSPYVF STTPESITEK SSIATMTSVG KSRTTTESSA CESASDKPVS PQAKGTPKEI TPREDIDEDS SDPSFNPMYA KEIFSYMKER EEQFILTDYM NRQIEITSDM RAILVDWLVE VQVSFEMTHE TLYLAVKLVD LYLMKAVCKK DKLQLLGATA FMIAAKFEEH NSPRVDDFVY ICDDNYQRSE VLSMEINILN VLKCDINIPI AYHFLRRYAR CIHTNMKTLT LSRYICEMTL QEYHYVQEKA SKLAAASLLL ALYMKKLGYW VPFLEHYSGY SISELHPLVR QLNKLLTFSS YDSLKAVYYK YSHPVFFEVA KIPALDMLKL EEILNCDCEA QGLVL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Cyclin B3 (CCNB3)

Andere Bezeichnung: CCNB3 (CCNB3 Produkte)

Synonyme: CG5814 Protein, Cyc B3 Protein, Dmel\\CG5814 Protein, anon-WO0118547.414 Protein, cycB3 Protein, l(3)L6540 Protein, ccnb3-a Protein, zgc:153369 Protein, MGC154490 Protein, NV16541 Protein, CCNB3 Protein, CYCB3 Protein, RGD1564367 Protein, Cyclin B3 Protein, cyclin B3 S homeolog Protein, cyclin B3 Protein, cyclin B3 L homeolog Protein, G2/mitotic-specific cyclin-B3 Protein, CycB3 Protein, ccnb3.S Protein, ccnb3 Protein, ccnb3.L Protein, LOC5572162 Protein, LOC100122822 Protein, CCNB3 Protein, LOC100394742 Protein, Ccnb3 Protein

Hintergrund: G2/mitotic-specific cyclin-B3,FUNCTION: Cyclins are positive regulatory subunits of the cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I. {ECO:0000269|PubMed:12185076}.

Molekulargewicht: 157.9 kDa

UniProt: Q8WWL7

Pathways: Zellzyklus, Chromatin Binding