Angiopoietin 1 Protein (ANGPT1) (AA 20-498)

Produktnummer ABIN2816939

Dieses Recombinant Angiopoietin 1-Protein wird in Mammalian Cells exprimiert.

Kurzübersicht für Angiopoietin 1 Protein (ANGPT1) (AA 20-498) (ABIN2816939)

Target: Angiopoietin 1 (ANGPT1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Mammalian Cells

Applikation: Functional Studies (Func)

Reinheit: >90 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 20-498

Sequenz: Ser20 - Phe498, Accession # Q5HYA0

Produktmerkmale: Biological Activity: The ED50, as determined by inhibiting serum deprivation induced apoptosis in human HUVEC, is less than 50 ng/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody.

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris-Citrate, pH 6.5, 500 mM NaCl

Lagerung: -20 °C

Antigendetails

Target: Angiopoietin 1 (ANGPT1)

Andere Bezeichnung: ANGPT1

Hintergrund: Relevance: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. {ECO:0000269|PubMed:15284220, ECO:0000269|PubMed:18425119, ECO:0000269|PubMed:18425120, ECO:0000269|PubMed:9204896}.
Synonyms: ANG-1,

Molekulargewicht: 56.3 kDa

UniProt: Q15389

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process