TDO2 Protein (His tag)

Produktnummer ABIN2745861

Produktdetails

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Protein-Typ: Recombinant

Spezies: Anopheles gambiae

Quelle: Synthetic

Aufreinigungstag / Konjugat: Dieses TDO2 Protein ist gelabelt mit His tag.

Verwendungszweck: TDO (Anopheles gambiae) (rec.) (His)

Produktmerkmale: Full-length TDO (Anopheles gambiae) is fused at the N-terminus to a His-tag.

Reinheit: >98 % (SDS-PAGE)

Biological Activity Comment: Specific activity towards L-Trp: 87.0μmol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: 0.5 mg/mL (Lot specific)

Konzentration: Lot specific

Buffer: In 50 mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10 % glycerol.

Handhabung: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Haltbarkeit: 6 months

Antigendetails

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Andere Bezeichnung: TDO (TDO2 Produkte)

Hintergrund:

TPH2, TRPO, Tryptophan 2,3-dioxygenase, Tryptophan Oxygenase, Tryptophan Pyrrolase, Tryptophanase

Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin, in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.

Molekulargewicht: ~47.9kDa

UniProt: O77457