WISP1 Protein (Transcript Variant 1)
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- Target Alle WISP1 Proteine anzeigen
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- Transcript Variant 1
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human WISP1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin-Niveau
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
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- Discover our top product WISP1 Protein
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handhabung
- Resuspend the protein in the desired concentration in proper buffer
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- Andere Bezeichnung
- Wisp1 (WISP1 Produkte)
- Hintergrund
- This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
- Molekulargewicht
- 38.1 kDa
- NCBI Accession
- NP_003873
- Pathways
- WNT Signalweg, Growth Factor Binding
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