Thrombopoietin Protein (THPO) (Transcript Variant 1)

Produktnummer ABIN2733599

Recombinant Thrombopoietin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Thrombopoietin Protein (THPO) (Transcript Variant 1) (ABIN2733599)

Target: Thrombopoietin (THPO)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Proteineigenschaft: Transcript Variant 1

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Thrombopoietin (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thrombopoietin

Hintergrund: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 18.6 kDa

NCBI Accession: NP_000451

Pathways: JAK-STAT Signalweg, Hormone Activity