SMAD7 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2732224

Dieses Recombinant SMAD7-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für SMAD7 Protein (Myc-DYKDDDDK Tag) (ABIN2732224)

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses SMAD7 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Produktmerkmale:

• Recombinant human SMAD7 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Andere Bezeichnung: Smad7

Hintergrund: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 46.2 kDa

NCBI Accession: NP_005895

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization