PDGFB Protein

Produktnummer ABIN2728540

Dieses Recombinant PDGFB-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für PDGFB Protein (ABIN2728540)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human PDGFB protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Andere Bezeichnung: Pdgfb

Molekulargewicht: 24.4 kDa

NCBI Accession: NP_035187

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling