PAK4 Protein (Transcript Variant 1)

Produktnummer ABIN2728177

Dieses Recombinant PAK4-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für PAK4 Protein (Transcript Variant 1) (ABIN2728177)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Reinheit: > 90 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Proteineigenschaft: Transcript Variant 1

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human PAK4 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Specific activity was determined as 5,595 pmoles/min/μg, according to the Zlyte assay protocol

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Andere Bezeichnung: Pak4

Hintergrund: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molekulargewicht: 33.3 kDa

NCBI Accession: NP_005875

Pathways: RTK Signalweg