MMP2 Protein (Transcript Variant 1)

Produktnummer ABIN2726159

Recombinant MMP2-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für MMP2 Protein (Transcript Variant 1) (ABIN2726159)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Reinheit: > 90 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Proteineigenschaft: Transcript Variant 1

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human MMP-2 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: MMP-2 activity was measured by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. At an MMP-2 concentration of 2.5 ug/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: Mmp-2

Hintergrund: This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 62 kDa

NCBI Accession: NP_004521

Pathways: Activation of Innate immune Response