IL-4 Protein (Transcript Variant 1)

Produktnummer ABIN2723725

Dieses Recombinant IL-4-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für IL-4 Protein (Transcript Variant 1) (ABIN2723725)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: Transcript Variant 1

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-4 / IL4 (transcript variant 1) protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 2 - 4 ng/ml, corresponding to a specific activity of 2.5 - 5 x 10^5 units/mg, determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 1 x PBS

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: Interleukin-4,il4

Molekulargewicht: 13.3 kDa

NCBI Accession: NP_067258

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation