IL-4 Protein (Transcript Variant 1)

Produktnummer ABIN2723723

Recombinant IL-4-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL-4 Protein (Transcript Variant 1) (ABIN2723723)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Proteineigenschaft: Transcript Variant 1

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-4 / IL4 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is > 2.0 ng/ml, corresponding to a specific activity of > 5 x 10^5 units/mg.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: Interleukin-4,il4

Molekulargewicht: 13.5 kDa

NCBI Accession: NP_067258

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation