IL12B Protein

Produktnummer ABIN2723546

Recombinant IL12B-Protein exprimiert in Insect cells (Sf9).

Kurzübersicht für IL12B Protein (ABIN2723546)

Target: IL12B (Interleukin 12 beta (IL12B))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Insect cells (Sf9)

Applikation: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Reinheit: 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin 12b (Il12b) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.10 to 0.20 ng/ml, corresponding to a specific activity of 1.0 - 0.5 x 10^7 units/mg, determined by the dose dependent stimulation of IFN&gamma production by mouse splenocytes.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL12B (Interleukin 12 beta (IL12B))

Andere Bezeichnung: Interleukin 12b (Il12b)

Molekulargewicht: 57.5 kDa

NCBI Accession: NP_032378

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation