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GREM1 Protein (Transcript Variant 1)

Recombinant GREM1-Protein exprimiert in CHO Cells.
Produktnummer ABIN2722232

Kurzübersicht für GREM1 Protein (Transcript Variant 1) (ABIN2722232)

Target

Alle GREM1 Proteine anzeigen
GREM1 (Gremlin 1 (GREM1))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 13
  • 4
  • 3
  • 3
  • 1
  • 1
Human

Quelle

  • 13
  • 4
  • 4
  • 2
  • 1
  • 1
CHO Cells

Applikation

Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Reinheit

> 90 % as determined by SDS-PAGE and Coomassie blue staining
  • Proteineigenschaft

    Transcript Variant 1

    Spezifität

    Optimal preservation of protein structure, post-translational modifications and functions.

    Produktmerkmale

    • Recombinant human Gremlin-1 / GREM1 (transcript variant 1) protein expressed in CHO cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    Endotoxin-Niveau

    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

    Biological Activity Comment

    Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11ug/mL.
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  • Applikationshinweise

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

    Handhabung

    Resuspend the protein in the desired concentration in proper buffer

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target

    GREM1 (Gremlin 1 (GREM1))

    Andere Bezeichnung

    Gremlin-1,grem1

    Hintergrund

    This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

    Molekulargewicht

    18.3 kDa

    NCBI Accession

    NP_037504

    Pathways

    Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location
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