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BDNF Protein (Transcript Variant 6)

Dieses Recombinant BDNF-Protein wird in Escherichia coli (E. coli) exprimiert.
Produktnummer ABIN2715217

Kurzübersicht für BDNF Protein (Transcript Variant 6) (ABIN2715217)

Target

Alle BDNF Proteine anzeigen
BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 22
  • 8
  • 7
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Quelle

  • 33
  • 17
  • 3
  • 2
  • 1
  • 1
Escherichia coli (E. coli)

Applikation

Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Reinheit

> 95 % as determined by SDS-PAGE and Coomassie blue staining
  • Proteineigenschaft

    Transcript Variant 6

    Spezifität

    Optimal preservation of protein structure, post-translational modifications and functions.

    Produktmerkmale

    • Recombinant human BDNF (transcript variant 6) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    Endotoxin-Niveau

    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

    Biological Activity Comment

    Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0ug/mL.
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  • Applikationshinweise

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

    Handhabung

    Resuspend the protein in the desired concentration in proper buffer

    Lagerung

    -80 °C

    Informationen zur Lagerung

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target

    BDNF (Brain-Derived Neurotrophic Factor (BDNF))

    Andere Bezeichnung

    Bdnf

    Hintergrund

    This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.

    Molekulargewicht

    27 kDa

    NCBI Accession

    NP_733930

    Pathways

    RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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