CYP7A1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2712786

Dieses Recombinant CYP7A1-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CYP7A1 Protein (Myc-DYKDDDDK Tag) (ABIN2712786)

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Standard (STD)

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Produktdetails

Aufreinigungstag / Konjugat: Dieses CYP7A1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Produktmerkmale:

• Recombinant human CYP7A1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Andere Bezeichnung: Cyp7a1

Hintergrund: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis.

Molekulargewicht: 57.5 kDa

NCBI Accession: NP_000771

Pathways: Steroid Hormone Biosynthesis, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha