Caspase 3 Protein (CASP3)

Produktnummer ABIN2688833

Recombinant Caspase 3-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Caspase 3 Protein (CASP3) (ABIN2688833)

Target: Caspase 3 (CASP3)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Fluorescence Spectroscopy (FS)

Reinheit: ≥ 95%

Produktdetails

Marke: BD Pharmingen™

Aufreinigung: Active caspase-3 was expressed in E.coli and purified. When expressed in E. coli, caspase-3 will spontaneously undergo autoprocessing to yield the subunits characteristic of the active enzyme. Cleavage of the Ac-DEVD-AMC substrate by recombinant human caspases-3, -6, -7, and -8. The activity of the caspases was analyzed by spectrofluorometry using an excitation at 380 nm and an emission wwavelength of 430-460 nm (peak is at 440 nm).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

BD Pharmingen™ Recombinant Human Active Caspase-3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 50 mM Tris ( pH 8.0) with 100 mM NaCl and 50 mM imidazole

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. The thawed active enzyme is generally stable at 4°C for about one week.

Antigendetails

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase-3

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation