KDM3B Protein (DYKDDDDK Tag)
-
- Target Alle KDM3B Proteine anzeigen
- KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))
- Protein-Typ
- Recombinant
-
Spezies
- Human
-
Quelle
- Baculovirus
- Aufreinigungstag / Konjugat
- Dieses KDM3B Protein ist gelabelt mit DYKDDDDK Tag.
- Applikation
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Produktmerkmale
- Recombinant JMJD1B / KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 193.4 kDa.
- Top Product
- Discover our top product KDM3B Protein
-
Lysine (K)-Specific Demethylase 3B (KDM3B) (AA 1-759) protein (GST tag)
KDM3B Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Lysine (K)-Specific Demethylase 3B (KDM3B) (AA 2-1562) protein (His tag)Crystallography grade KDM3B Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
-
- Applikationshinweise
- Recombinant JMJD1B / KDM3B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 40 nM Recombinant JMJD1B / KDM3B protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Handhabung
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
-
- Target
- KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))
- Andere Bezeichnung
- JMJD1B / KDM3B (KDM3B Produkte)
- Synonyme
- KDM3B Protein, JMJD1B Protein, 5830462I21Rik Protein, JHDM2B Protein, Jmjd1b Protein, mKIAA1082 Protein, 5qNCA Protein, C5orf7 Protein, NET22 Protein, lysine demethylase 3B Protein, lysine (K)-specific demethylase 3B Protein, lysine-specific demethylase 3B Protein, KDM3B lysine (K)-specific demethylase 3B Protein, KDM3B Protein, LOC100540242 Protein, kdm3b Protein, Kdm3b Protein
- Hintergrund
- Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.
- Molekulargewicht
- 193.4 kDa
- Pathways
- Warburg Effekt
-
