KDM3B Protein (DYKDDDDK Tag)

Produktnummer ABIN2669679

Recombinant KDM3B-Protein exprimiert in Baculovirus.

Kurzübersicht für KDM3B Protein (DYKDDDDK Tag) (ABIN2669679)

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktdetails

Aufreinigungstag / Konjugat: Dieses KDM3B Protein ist gelabelt mit DYKDDDDK Tag.

Produktmerkmale: Recombinant JMJD1B / KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 193.4 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant JMJD1B / KDM3B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 40 nM Recombinant JMJD1B / KDM3B protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Andere Bezeichnung: JMJD1B / KDM3B

Hintergrund: Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.

Molekulargewicht: 193.4 kDa

Pathways: Warburg Effekt