Thrombopoietin Protein (THPO) (AA 22-195)

Produktnummer ABIN2667585

Recombinant Thrombopoietin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Thrombopoietin Protein (THPO) (AA 22-195) (ABIN2667585)

Target: Thrombopoietin (THPO)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Biochemical Assay (BCA)

Reinheit: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Produktdetails

Proteineigenschaft: AA 22-195

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: The expected ED50 is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg, as determined by the dose-dependent stimulation of the proliferation of human MO7e cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thrombopoietin

Hintergrund: Mouse Thrombopoietin (TPO) is a protein consisting of an N-terminus domain with homology to erythropoietin (EPO) and a C-terminus domain with multiple N-linked and O-linked glycosylation sites. TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. It signals through the c-mpl receptor and stimulates the proliferation and maturation of megakaryocytes, hence, TPO plays an important role in regulating the level of circulating platelets in vivo. It has also been shown that TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitors. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Molekulargewicht: The 174 amino acid recombinant protein has a predicted molecular mass of 18.7 kDa. The predicted N-terminal amino acid is Ser.

Pathways: JAK-STAT Signalweg, Hormone Activity