IL-9 Protein (AA 19-144)

Produktnummer ABIN2667544

Produktdetails

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 19-144

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC)

Reinheit: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0 x 106 units/mg as determined by the dose-dependent stimulation of the proliferation of human M07e cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Andere Bezeichnung: IL-9 (IL9 Produkte)

Synonyme: IL9 Protein, IL-9 Protein, Il-9 Protein, P40 Protein, HP40 Protein, interleukin 9 Protein, IL9 Protein, il9 Protein, Il9 Protein

Hintergrund: IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα ) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.

Molekulargewicht: The 127 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.2 kDa. The predicted N-terminal amino acid is Met.

Pathways: JAK-STAT Signalweg