IL-22 Protein (AA 34-179, N-Term)

Produktnummer ABIN2667506

Dieses Recombinant IL-22-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-22 Protein (AA 34-179, N-Term) (ABIN2667506)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS)

Reinheit: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Produktdetails

Proteineigenschaft: N-Term, AA 34-179

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.7 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 1.4 x 106 units/mg as determined by the dose-dependent stimulation of human Colo205 cells in production of IL-10.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Andere Bezeichnung: IL-22

Hintergrund: IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79 % amino acid identity with mouse IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.

Molekulargewicht: The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.9 kDa. The predicted N-terminal amino acid is Met.