IL-22 Protein (AA 34-179) (His tag)

Produktnummer ABIN7320589

Recombinant IL-22-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL-22 Protein (AA 34-179) (His tag) (ABIN7320589)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 34-179

Aufreinigungstag / Konjugat: Dieses IL-22 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Interleukin-22/IL-22 Protein

Sequenz: Leu34-Val179

Produktmerkmale: Recombinant Mouse Interleukin-22 is produced by our E.coli expression system and the target gene encoding Leu34-Val179 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce IL-10 secretion in COLO205 cells. The ED₅₀ for this effect is <0.3 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Andere Bezeichnung: Interleukin-22/IL-22

Hintergrund: Interleukin,Cytokine Zcyto,IL-D,TIFIL,Cytokine Zcyto18,IL-10-related T-cell-derived-inducible factor,IL-22a,IL-D110,IL-TIF,Interleukin-22a,TIFa,TIFIL-23,UNQ3099/PRO10096,zcyto18,Interleukin-22,T-cell-derived-inducible,factor(IL-TIF),Interleukin-22a(IL-22a),IL-TIF alpha,IL-10-related,IL-22,Il22a,Iltif,Iltifa,Il22,TIFa,Interleukin-22 (IL-22) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acid signal peptide that is cleaved to generate a 147 amino acid mature protein that shares approximately 79 % and 22 % sequence identity with human IL22 and IL10, respectively. IL22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and up-regulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.

Molekulargewicht:

Calculated MW: 17.6 kDa

Observed MW: 17 kDa

Gen-ID: 50929

UniProt: Q9JJY9