FGF2 Protein (AA 11-154)

Produktnummer ABIN2667409

Dieses Recombinant FGF2-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für FGF2 Protein (AA 11-154) (ABIN2667409)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Multiplex Assay (MA)

Reinheit: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 11-154

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.3 to 2 ng/ml, corresponding to a specific activity of 3.3 to 0.5 x 106 units/mg, as determined by the dose dependent stimulation of 3T3 cell proliferation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, 1 mM DTT, 0.5 mM EDTA.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: FGF-Basic

Hintergrund: FGFb is a member of the fibroblast growth factor (FGF) family which includes 23 members. FGFb is expressed in almost all tissues and play important roles in a variety of normal and pathological processes, including development, wound healing and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. FGFb shows potent angiogenic activity and has been implicated in tumor angiogenesis. FGF-2 significantly promoted the proliferation of adipose-derived mesenchymal cells (AMC) and enhanced chondrogenesis in three-dimensional micromass culture. FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4. In addition, FGFb binds to the ECM, and heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface, although there is controversy regarding the exact manner by which this occurs.

Molekulargewicht: The 144 amino acid recombinant protein has a predicted molecular mass of 16,223 Da. The DTT-reduced and non-reduced protein migrate at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Alanine.

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine