CXCL14 Protein (AA 35-111)

Produktnummer ABIN2666914

Recombinant CXCL14-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CXCL14 Protein (AA 35-111) (ABIN2666914)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: ELISA, Flow Cytometry (FACS)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 35-111

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract PGE2 activated THP-1 cells in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Andere Bezeichnung: CXCL14

Hintergrund: CXCL14 was initially cloned from breast and kidney cells, and it is expressed at high levels and ubiquitously in normal tissue. CXCL14 is well conserved between humans, birds, frogs and fish at the amino acid level, and human and mouse CXCL14 share 95 % identity. Original studies showed a decrease in the expression of CXCL14 in head and neck squamous cell carcinoma (HNSCC) and cervical squamous cell carcinoma (SCCs) tumors, and the induction of expression of CXCL14 in human oral carcinoma prevents tumor growth of these cells in vivo. Therefore, it has been suggested that CXCL14 possesses tumor suppressing function, and it has been speculated that CXCL14 secreted by stromal cells chemoattracts iDCs (immature dendritic cells) and NK cells that activate the immune response against tumor cells. Most recent data showed that epigenetic silencing of CXCL5, CXCL12, and CXCL14 occurs in 75 % of primary lung adenocarcinomas. Consequently, the low expression of CXCL14 in tumors might allow them to escape immune surveillance. Opposite of HNSCC and SCCs tumors, prostate, pancreatic, and colorectal cancers show high expression of CXCL14, inducing growth and invasiveness of pancreatic and breast cancer cells.

Molekulargewicht: The 77 amino acid recombinant protein has a predicted molecular mass of approximately 9.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 13 and 14 kDa by SDS-PAGE respectively. The N-terminal amino acid is Met.

Pathways: Autophagie