CCL24 Protein (AA 27-119)

Produktnummer ABIN2666899

Dieses Recombinant CCL24-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für CCL24 Protein (AA 27-119) (ABIN2666899)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 27-119

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract BaF3-mCCR3 transfectants in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Andere Bezeichnung: CCL24

Hintergrund: CLL24 was initially cloned from a library of activated macrophages, and using human eosinophils, it was shown that it binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). In addition, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7. Eotaxin/CCR3 expression has been studied extensively in the pathogenesis of asthma and allergy. In addition, the eotaxin/CCR3 axis has been associated to additional inflammatory and autoimmune disorders including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis. CCL24 has also been associated to neovascular age related macular degeneration.

Molekulargewicht: The 93 amino acid recombinant protein has a predicted molecular mass of approximately 10.4 kDa. The DTT-reduced protein migrates at approximately 12 kDa and non-reduced protein migrates at 14 kDa by SDS-PAGE. The N-terminal amino acid is Valine.

Pathways: Regulation of Actin Filament Polymerization