IL-9 Protein (AA 19-144)

Produktnummer ABIN2666758

Dieses Recombinant IL-9-Protein wird in Insektenzellen exprimiert.

Kurzübersicht für IL-9 Protein (AA 19-144) (ABIN2666758)

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Insektenzellen

Applikation: Flow Cytometry (FACS)

Reinheit: > 90 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 19-144

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.1 EU per μg of protein as determine by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Human IL-9 induces proliferation of MO7e cells. ED50=0.1-0.5 ng/mL, corresponding to a specific activity of 2-10 x 106 units/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Andere Bezeichnung: IL-9

Hintergrund: IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα ) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.

Molekulargewicht: The 126 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa. The protein migrates as multiple bands with the molecular mass of 18 - 25 kDa band in SDS-PAGE in DTT-reducing conditions due to different glycosylation. The N-

Pathways: JAK-STAT Signalweg