MMP2 Protein (AA 34-662, C-Term)

Produktnummer ABIN2666505

Dieses Recombinant MMP2-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für MMP2 Protein (AA 34-662, C-Term) (ABIN2666505)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: C-Term, AA 34-662

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.1 EU per μg of protein as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Mouse MMP-2 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2 with an activity above 1400 pmol/min/μg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening.

Buffer: 0.22 μm filtered protein solution is in TCN (25 mM TRIS, 5 mM CaCl2, 150 mM NaCl, pH 7.5).

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -70°C for six months.

Antigendetails

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: MMP-2

Hintergrund: MMP-2, also known as gelatinase A, is a member of matrix metalloproteinase family proteins (MMPs). MMPs are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling as well as in disease processes such as arthritis and metastasis. MMP-2 consists of a prodomain, which is cleaved upon activation, a catalytic domain containing the zinc binding site, a fibronectin-like domain (that plays a role in the substrate targeting), and a carboxyl terminal (hemopexin-like repeats) domain. Activation of MMP-2 requires proteolytic processing: a complex of membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP-2 from the extracellular milieu to the cell surface. Next, the MMP-2 is activated by active MT1-MMP and subsequently undergoes auto-catalytic cleavage. Substrates of MMP-2 include type IV collagen, aggrecan, link protein, decorin, fibronectin, and type X and XI collagens, all of which are components of the articular cartilaginous matrix. Importantly, MMP-2 secretion is elevated in several types of human cancers and its elevated expression has been associated with poor prognosis. Mutations in the MMP2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. MMP-2 deficient mice exhibit slightly delayed growth, reduced neovascularization, retarded tumor progression, an exaggerated asthma response to allergens, and impaired branching morphogenesis of the mammary gland.

Molekulargewicht: This 663 amino acid recombinant protein has a predicted molecular mass of approximately 74.5 kDa. The protein migrates at about 72 kDa in DTT-reducing conditions and about 71 kDa in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid i

Pathways: Activation of Innate immune Response