E-cadherin (CDH1)
(Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Proteineigenschaft
AA 23-621
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses E-cadherin Protein ist gelabelt mit Fc Tag.
Sequenz
AA 23-621
Produktmerkmale
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 92.4 kDa. As a result of different glycosylation, the protein migrates as 110-125 kDa Cadherin, a 48 kDa Propeptide under reducing (R) condition, and 200-250 kDa under non-reducing (NR) condition (SDS-PAGE).
Crystallography grade
CDH1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Crystallography grade
CDH1
Spezies: Maus
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
50 mM Tris, 100 mM Glycine, pH 7.5
Handhabung
Please avoid repeated freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Eshghi, Gaultney, England, Brûlé, Miras, Sato, Coburn, Bellalou, Moriarty, Haouz, Picardeau: "An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins." in: Cellular microbiology, Vol. 21, Issue 2, pp. e12949, (2019) (PubMed).
Campbell, Salvi, OBrien, Superfine, DeMali: "PAK2 links cell survival to mechanotransduction and metabolism." in: The Journal of cell biology, Vol. 218, Issue 6, pp. 1958-1971, (2019) (PubMed).
Suffoletto, Jetta, Hua: "E-cadherin mediated lateral interactions between neighbor cells necessary for collective migration." in: Journal of biomechanics, Vol. 71, pp. 159-166, (2019) (PubMed).
Target
E-cadherin (CDH1)
(Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).