VEGF Protein (AA 27-191) (Biotin)

Produktnummer ABIN2181906

Produktdetails

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 27-191

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses VEGF Protein ist gelabelt mit Biotin.

Marke: MABSol®,UltraLys

Sequenz: AA 27-191

Spezifität: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Produktmerkmale: The product does NOT contain any epitope tags. The protein has a calculated MW of 19 kDa (monomer). The protein migrates as 24 kDa (monomer) on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 43-50 kDa under non-reducing (NR) condition.

Reinheit: >95 % as determined by reduced SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Referenzen

McGonigle, Majumder, Kolber-Simonds, Wu, Hart, Noland, TenDyke, Custar, Li, Du, Postema, Lai, Twine, Woodall-Jappe, Nomoto: "Neuropilin-1 drives tumor-specific uptake of chlorotoxin." in: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 67, (2019) (PubMed).

Antigendetails

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Andere Bezeichnung: VEGF (VEGF Produkte)

Hintergrund: VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.

Molekulargewicht: 19.2 kDa

NCBI Accession: NP_001165097